Journal: International Journal of Oncology

Article Title: Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo

doi: 10.3892/ijo.2017.4183

Figure Lengend Snippet: Rg3 represses the growth and migration of colorectal cancer (CRC) cells. (A) Exposure to various concentrations of Rg3 resulted in a dose- and time-dependent growth inhibition on LoVo, SW620 and HCT116 CRC cells. (B) Treatment with 200 μ M Rg3 resulted in time-dependent inhibition on the migration of LoVo and SW620 cells. * P<0.05 and ** P<0.01 indicate significant differences compared with the respective control groups.

Article Snippet: The human CRC cell lines, LoVo, SW620 and HCT116, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration, Inhibition, Control

Journal: International Journal of Oncology

Article Title: Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo

doi: 10.3892/ijo.2017.4183

Figure Lengend Snippet: Rg3 represses the stemness of colorectal cancer (CRC) cells. (A) Rg3 repressed the expressions of CD24, CD44 and EpCAM at the mRNA level in LoVo cells. Cells were treated with 200 μ M Rg3 and mRNA expressions were determined using real-time PCR. (B–D) Rg3 reducd the levels of CD24 (B), CD44 (C) and EpCAM (D) proteins in LoVo cells. Cells were treated with 200 μ M Rg3 and protein levels were evaluated using flow cytometry. (E) Rg3 treatment (200 μ M) decreased the population of CD24 + /CD44 + /EpCAM + cells among LoVo cells. (F and G) Rg3 treatment inhibited the clone formation ability of LoVo (F) and HCT116 (G) cells in a dose-dependent manner. * P<0.05 and ** P<0.01 indicate significant differences compared with the respective control groups.

Article Snippet: The human CRC cell lines, LoVo, SW620 and HCT116, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Real-time Polymerase Chain Reaction, Flow Cytometry, Control

Journal: International Journal of Oncology

Article Title: Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo

doi: 10.3892/ijo.2017.4183

Figure Lengend Snippet: Rg3 represses growth and stemness of colorectal cancer (CRC) cells in vivo . (A) Images of resected LoVo and SW620 orthotopic xenografts. Mice bearing LoVo or SW620 orthotopic xenografts were randomly divided into two groups (five mice in each group): The Rg3 group, which received Rg3 by gastric perfusion daily (25 mg/kg) for 12 consecutive days, and the control group, which was treated with normal saline. (B) Tumor weights of resected LoVo and SW620 orthotopic xenografts. (C–J) Immunohistochemical examination of Ki-67 (C and D), CD24 (E and F), CD44 (G and H) and EpCAM (I and J) levels in LoVo and SW620 orthotopic xenografts. * P<0.05 and ** P<0.01 indicate significant differences compared with the respective control groups.

Article Snippet: The human CRC cell lines, LoVo, SW620 and HCT116, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: In Vivo, Control, Saline, Immunohistochemical staining

Journal: International Journal of Oncology

Article Title: Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo

doi: 10.3892/ijo.2017.4183

Figure Lengend Snippet: Rg3 represses angiogenesis of colorectal cancer (CRC). (A) Immunohistochemical examination of LoVo and SW620 orthotopic xenografts using an anti-CD34 antibody. (B) Rg3 treatment decreased the microvessel density (MVD) levels in both LoVo and SW620 orthotopic xenografts. (C) Real-time PCR showing that Rg3 downregulated the expression of angiogenesis-related genes in LoVo cells. * P<0.05 and ** P<0.01 indicate significant differences compared with the respective control groups.

Article Snippet: The human CRC cell lines, LoVo, SW620 and HCT116, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Immunohistochemical staining, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: International Journal of Oncology

Article Title: Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo

doi: 10.3892/ijo.2017.4183

Figure Lengend Snippet: Rg3 downregulates the levels of B7-H1 and B7-H3 in colorectal cancer (CRC). (A and B) Immunohistochemical examinations of B7H1 (A) and B7H3 (B) levels in human CRC tissue samples. (C and D) Kaplan-Meier curves for CRC overall survival according to tumor B7-H1 (C) and B7-H3 (D) expression status (low vs. high, P<0.01). (E and F) Immunohistochemical examination of B7H1 (E) and B7H3 (F) levels in LoVo orthotopic xenografts after Rg3 treatment.

Article Snippet: The human CRC cell lines, LoVo, SW620 and HCT116, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Immunohistochemical staining, Expressing

Journal: Journal of Korean Medical Science

Article Title: Dual-Blocking of PI3K and mTOR Improves Chemotherapeutic Effects on SW620 Human Colorectal Cancer Stem Cells by Inducing Differentiation

doi: 10.3346/jkms.2016.31.3.360

Figure Lengend Snippet: Stemness properties of sorted SW620 CD133+ cells. ( A ) The mRNA expression of stemness and differentiation marker in SW620 CD133+ and CD133–cells were measured by RT-PCR. β-Actin was used as a loading control. For comparisons, the relative value for markers of CD133–cells was considered to be “1”. ( B ) Self-renewal ability of sorted cells was analyzed by sphere formation assay. Pictures were taken at ×40 magnification. Scale bar = 100 µm. Data are expressed as the mean ± standard error of the mean (SEM) of three independent experiments performed (* P < 0.001).

Article Snippet: The SW620 human colorectal cancer cell line was purchased from the Korean cell line bank (Seoul, Korea).

Techniques: Expressing, Marker, Reverse Transcription Polymerase Chain Reaction, Control, Tube Formation Assay

Journal: Journal of Korean Medical Science

Article Title: Dual-Blocking of PI3K and mTOR Improves Chemotherapeutic Effects on SW620 Human Colorectal Cancer Stem Cells by Inducing Differentiation

doi: 10.3346/jkms.2016.31.3.360

Figure Lengend Snippet: The drug sensitivity of inhibited SW620 CD133+ cells. ( A ) Inhibited SW620 CD133+ cells were treated with paclitaxel (100 nM) for 24 hours and cell viability was assessed by CCK-8 assay. For comparisons cell viability of DMSO treated CD133+ cells was 100%. ( B ) Self-renewal ability of inhibited SW620 CD133+ cells treated with paclitaxel and self-renewal ability was assessed by sphere formation assay. ( C ) Differentiation marker (CEA) expression of inhibited CSCs treated with paclitaxel. The protein expression of CEA was assessed by IF. For the comparison, relative intensity of CTCF for CEA of DMSO treated CD133+ cells was defined as “1”. All data are expressed as the mean ± standard error of the mean (SEM) of three independent experiments performed (* P < 0.05, † P < 0.01, ‡ P < 0.001, “N/S” means “statistically not significant”). Pictures were taken at ×400 magnification. Scale bar = 10 μm.

Article Snippet: The SW620 human colorectal cancer cell line was purchased from the Korean cell line bank (Seoul, Korea).

Techniques: CCK-8 Assay, Tube Formation Assay, Marker, Expressing, Comparison

Journal: Journal of Korean Medical Science

Article Title: Dual-Blocking of PI3K and mTOR Improves Chemotherapeutic Effects on SW620 Human Colorectal Cancer Stem Cells by Inducing Differentiation

doi: 10.3346/jkms.2016.31.3.360

Figure Lengend Snippet: Xenograft tumorigenecity assay of inhibitors treated SW620 CD133+ cells before and after injection of anti-cancer drug. ( A ) Comparison of tumorigenecity of inhibitor treated SW620 CD133+ cells in BALB/ c nude mice. Balb/ c nude mice were subcutaneously injected with sorted SW620 CD133+ cells. The left flank was injected with untreated CD133+ cells, while the right flank was injected with CD133+ cells treated with each inhibitor. Anti-cancer drug (paclitaxel) was injected since 28 days. ( B ) Tumor volume (mm 3 ) of xenografts from Balb/ c mice. The change in tumor volume was checked for each group. DMSO treated group was used as a control. Data are expressed as the mean ± standard error of the mean (SEM) of mice for each group.

Article Snippet: The SW620 human colorectal cancer cell line was purchased from the Korean cell line bank (Seoul, Korea).

Techniques: Injection, Comparison, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O -glycosylated MUC1 via PI3K/AKT pathway

doi: 10.1186/s13046-018-0994-x

Figure Lengend Snippet: Alteration of linc01296 affected the proliferation and migration of CRC cells. a CCK8 assay was carried out to measure the absorbance of transfected CRC cells. b Linc01296 affected colony formation ability of CRC cells. c Edu staining also depicted weaken proliferative ability with low linc01296 expression. Green fluorescence: Edu, blue fluorescence: DAPI. d Immunofluorescence staining of Ki67 was obtained by a fluorescence microscope after silinc01296 or vector treatment. Red fluorescence: Ki67, blue fluorescence: DAPI. e Weaken aggressiveness was seen in SW620 cells transfected with silinc01296 than the cells transfected with siSCR. f The viability of CRC cells transfected with silinc01296 was determined by CCK8 assay. g Enhanced linc01296 facilitated colony formation. h Edu staining proved high proliferation of CRC cells. Green fluorescence: Edu, blue fluorescence: DAPI. (I) Ki67 expression in the transfected CRC cells was observed by immunofluorescence. j Migratory and invasive ability was strengthened in SW480 transfected with linc01296. Data are the means ± SD of triplicate determinants (* P < 0.05)

Article Snippet: The human CRC cell lines SW620, SW480, HCT-8, 5-FU-resistant HCT-8 cells (HCT-8/5-FU) and LoVo were obtained from KeygenBiotech Co. Ltd. (Nanjing, China), which was recently authenticated as truly CRC cell lines.

Techniques: Migration, CCK-8 Assay, Transfection, Staining, Expressing, Fluorescence, Immunofluorescence, Microscopy, Plasmid Preparation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O -glycosylated MUC1 via PI3K/AKT pathway

doi: 10.1186/s13046-018-0994-x

Figure Lengend Snippet: The effect of co-expression linc01296 and miR-26a on CRC progression. a Flow cytometry showed O-linked glycosylation level detected by fluorescein isothiocyanate (FITC)-conjugated VVA on the cell surface of transfected CRC cells. b CCK8 assay showed variant growth rate of transfected CRC cells. c Colony formation assay detected proliferative formation with different treated CRC cells. d Aggressiveness changed was determined by transwell assay, the migratory and invasive cells were counted. e CRC cells were treated with 5-FU, and the cell viability was detected by CCK8 assay. f IC 50 values of treated CRC cells were calculated. g The effect of 5-FU on CRC cell proliferation was determined by colony formation assay. h Flow cytometry showed the apoptosis rate of transfected CRC cells in response to 5-FU. i FITC-VVA on transfected CRC cells surface was detected by flow cytometry. j The growth curves of transfected CRC cells were pictured after conducting CCK8 assay. k Proliferation of treated CRC cells was examined by colony formation assay. l Migration and invasion were observed in transfected SW620 cells. m With 5-FU treatment, the cell viability was determined by CCK8 assay. n The IC 50 values were subsequently calculated. o 5-FU resistant CRC cells were transfected with linc01296 or miR-26a mimic, and the colony formation was counted in response to 5-FU. p The apoptosis rate of transfected CRC cells was detected after 5-FU treatment by flow cytometry. The data were means ± SD of three independent assays (* P < 0.05)

Article Snippet: The human CRC cell lines SW620, SW480, HCT-8, 5-FU-resistant HCT-8 cells (HCT-8/5-FU) and LoVo were obtained from KeygenBiotech Co. Ltd. (Nanjing, China), which was recently authenticated as truly CRC cell lines.

Techniques: Expressing, Flow Cytometry, Glycoproteomics, Transfection, CCK-8 Assay, Variant Assay, Colony Assay, Transwell Assay, Migration

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O -glycosylated MUC1 via PI3K/AKT pathway

doi: 10.1186/s13046-018-0994-x

Figure Lengend Snippet: Linc01296 mediates liver metastasis and tumorigenesis of CRC cells in vivo. a CRC liver metastasis model were photographed of SW620 cells transfected with shSCR or shLinc01296 (up panel), and SW480 cells transfected with LV-NC and LV-Linc01296 (down panel). b H&E and IHC staining of liver and spleen derived from transfected SW620 cells (up panel) and transfected SW480 cells (down panel) were performed. c GFP signals of xenograft tumors were obtained with DMSO, 5-FU, 5-FU + LY294002 treated nude mice. d GALNT3 and Ki67 expression was shown by IHC staining. e Tumor weight of each group was detected. f Tumor volume was recorded

Article Snippet: The human CRC cell lines SW620, SW480, HCT-8, 5-FU-resistant HCT-8 cells (HCT-8/5-FU) and LoVo were obtained from KeygenBiotech Co. Ltd. (Nanjing, China), which was recently authenticated as truly CRC cell lines.

Techniques: In Vivo, Transfection, Immunohistochemistry, Derivative Assay, Expressing